Results support the recommendation that HIV-2 Western blot be included in the diagnostic algorithm for HIV-1/2 to followup negative or indeterminate HIV-1 Western blot results. HIV-1 seroconversion was observed in all 10 remaining samples during serological followup.Ĭonclusions: Two new HIV-2 seropositive cases were diagnosed using DAVIH–VIH-2 and HIV-2 Western blot in indeterminate HIV-1 Western blot samples. The two HIV-2–positive samples showed well defined reactivity to gp160, p53, p55 and p34 of HIV-1. All 12 ELISA-reactive samples belonged to the HIV-1 Western blot indeterminate group. Positive samples reacted against the p16, p26, gp36, p53, p56, p68 and gp105 proteins. Frequency, causes, and new challenges of indeterminate results in Western blot confirmatory testing for antibodies to human immunodeficiency virus. Western blot analysis of the 12 ELISA-reactive samples confirmed two positive samples (16.67%), 4 negative (33.33%) and 6 indeterminate (50%). The frequency of indeterminate results for both HSV-1 and HSV-2 may be rising possibly due to increased testing in the setting of declining seroprevalence. When I requested the records, I made the mistake and didnt stay with my file so the portion of the record with the indeterminate final result is missing.also, i have the first part of the western blot and it states that i have no bands observed. Results: Among all sera studied, HIV-2 ELISA identified 12 reactive serum samples (0.70%) and 1711 non-reactive (99.30%). The clinical and public health implication of HIV Western blot (WB) indeterminate results is yet to be appraised in sub-Saharan Africa, including Nigeria. saw it with my own eyes in doc office today. The epidemiological interview established by Cuba's National Program for Prevention and Control Sexually-Transmitted Diseases and HIV/AIDS was applied to HIV-2 Western blot-positive patients. Duplicate sera reactive by ELISA were confirmed by HIV-2 Western blot, results interpreted according to WHO criteria. Methods: HIV-2 reactivity was studied using the ELISA DAVIH–VIH-2 diagnostic kit (Cuba) in 1723 serum samples with negative or indeterminate results for HIV-1 Western blot from January 2005 through December 2008. Western Blot HIV-1 Western blot Indeterminate FIGURE 122-8 Algorithms for human immunodeficiency virus (HIV) detection in patients at risk for HIV infection. Objetive: Investigate the contribution of HIV-2 antibodies to negative or indeterminate HIV-1 Western blot results in serum samples from 2005 through 2008 in Cuba. Algorithm for clinical decisions related to positive, negative, and indeterminate findings from human immunodeficiency virus (HIV) Western blot (WB) tests. Indeterminate results constitute the main limitation of this test and HIV-2 infection is among their possible causes hence the importance of second-stage screening and confirmatory tests for HIV-2 infection. In Cuba, positive results in mixed HIV-1/2 screening assays are confirmed by HIV-1 Western blot. The Biotech/Du Pont Western blot kit thus frequently yields indeterminate test results in the absence of HIV-1 infection, the reproducibility of which is subject to lot-to-lot variability.Diagnosis, laboratory techniques and procedures, antibodies, HIV-2, Western blot, enzyme-linked immunosorbent assay, algorithm, Cuba.ĪBSTRACT Introduction: Differentiating between HIV-1 and HIV-2 infection is the first step to understanding HIV transmission, epidemiology and pathogenesis in geographical areas where both viruses circulate. ![]() There was 91% agreement in the test results of the first and second serum samples when the same lot of Western blot kit was used but only 36% agreement when different lots were used. No serum specimen collected after 2-11 months from individuals with indeterminate Western blot results was positive by EIA or Western blot. None of the subjects had a positive test result by any of these assays, but 32% had indeterminate Western blot tests, most of which demonstrated a single band of low intensity. However, indeterminate I/II), auto antibodies, pregnancy, abnormal determine the efficiency of kits to rule out non- Western blot reactivity to HIV-1 proteins may occur in individuals, who may not be bilirubin levels and in-vitro hemolysis have specific indeterminate (indeterminate result for infected with HIV. These specimens were screened for evidence of HIV-1 infection by enzyme immunoassay (EIA) and the Biotech/Du Pont Western blot (n = 168), culture (n = 122), and polymerase chain reaction assay (n = 20). As part of a phase 1 trial of a candidate AIDS vaccine, blood specimens were collected from 168 healthy adult volunteers at minimal or no risk for becoming infected with human immunodeficiency virus type 1 (HIV-1).
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